Selected Scientific Publications From 21^st Century Biochemicals' Customers
Custom Antibodies

* Denotes phosphospecific antibody

*Suppression of RhoG activity is mediated by a syndecan 4–synectin–RhoGDI1 complex and is reversed by PKCα in a Rac1 activation pathway. Arye Elfenbein, John M. Rhodes, Julia Meller, Martin A. Schwartz, Michiyuki Matsuda, and Michael Simons. J. Cell Biol., Jul 2009; 10.1083/jcb.200810179.

The Active Form of Human Aryl Hydrocarbon Receptor (AHR) Repressor Lacks Exon 8, and Its Pro¹⁸⁵ and Ala¹⁸⁵ Variants Repress both AHR and Hypoxia-Inducible Factor. Sibel I. Karchner, Matthew J. Jenny, Ann M. Tarrant, Brad R. Evans, Hyo Jin Kang, Insoo Bae, David H. Sherr, and Mark E. Hahn, Mol. Cell. Biol., Jul 2009; 29: 3465 – 3477

*Oligomeric resistin impairs insulin and AICAR-stimulated glucose uptake in mouse skeletal muscle by inhibiting GLUT4 translocation. Sebastian Beck Jørgensen, Jane Honeyman, Jonathan S. Oakhill, Daniel Fazakerley, Jacqueline Stöckli, Bruce E. Kemp, and Gregory R. Steinberg. Am J Physiol Endocrinol Metab, Jul 2009; 297: E57 - E66.

MURC/Cavin-4 and cavin family members form tissue-specific caveolar complexes
Michele Bastiani, Libin Liu, Michelle M. Hill, Mark P. Jedrychowski, Susan J. Nixon, Harriet P. Lo, Daniel Abankwa, Robert Luetterforst, Manuel Fernandez-Rojo, Michael R. Breen, Steven P. Gygi, Jorgen Vinten, Piers J. Walser, Kathryn N. North, John F. Hancock, Paul F. Pilch, and Robert G. Parton. J. Cell Biol., Jun 2009; 10.1083/jcb.200903053.

Translocation of SenP5 from the Nucleoli to the Mitochondria Modulates DRP1-dependent Fission during Mitosis. Rodolfo Zunino, Emélie Braschi, Liqun Xu, and Heidi M. McBride J. Biol. Chem., Jun 2009; 284: 17783 - 17795.

A truncation mutation in TBC1D4 in a family with acanthosis nigricans and postprandial hyperinsulinemia. Satya Dash, Hiroyuki Sano, Justin J. Rochford, Robert K. Semple, Giles Yeo, Caroline S. S. Hyden, Maria A. Soos, James Clark, Andrew Rodin, Claudia Langenberg, Celine Druet, Katherine A. Fawcett, Y. C. Loraine Tung, Nicolas J. Wareham, Inês Barroso, Gustav E. Lienhard, Stephen O'Rahilly, and David B. Savage. Proc. Natl. Acad. Sci., Jun 2009; 106: 9350 - 9355.

The active form of human aryl hydrocarbon receptor repressor lacks exon 8 and its Pro¹⁸⁵ and Ala¹⁸⁵ variants repress both AHR and HIF. Sibel I. Karchner, Matthew J. Jenny, Ann M. Tarrant, Brad R. Evans, Hyo Jin Kang, Insoo Bae, David H. Sherr, and Mark E. Hahn. Mol. Cell. Biol., Apr 2009;10.1128/MCB.00206-09

*A PP2A regulatory subunit regulates C. elegans insulin/IGF-1 signaling by modulating AKT-1 phosphorylation. Padmanabhan S, Mukhopadhyay A, Narasimhan SD, Tesz G, Czech MP, Tissenbaum HA. Cell, March 2009 136(5):939-51

*Disruption of Cdk5-Associated Phosphorylation of Residue Threonine-161 of the δ-Opioid Receptor: Impaired Receptor Function and Attenuated Morphine Antinociceptive Tolerance. Wei-Yan Xie, Yi He, Yan-Rui Yang, Ya-Fang Li, Kai Kang, Bao-Ming Xing, and Yun Wang. J. Neurosci., Mar 2009; 29: 3551 - 3564.

*Mechanism of activation and functional role of protein kinase Cη in human platelets. Yamini S. Bynagari, Bela Nagy Jr, Florin Tuluc, Kamala Bhavaraju, Soochong Kim, K. Vinod Vijayan, and Satya P. Kunapuli. J. Biol. Chem., Mar 2009; 10.1074/jbc.M808970200.

A regulatory role of the Bateman domain of IMP dehydrogenase in adenylate nucleotide biosynthesis. Maxim Pimkin, Julia Pimkina, and George D. Markham. J. Biol. Chem., Mar 2009; 284: 7960 - 7969.

*Hematopoietic Protein Tyrosine Phosphatase Mediates β₂-Adrenergic Receptor-Induced Regulation of p38 Mitogen-Activated Protein Kinase in B Lymphocytes. Jaclyn W. McAlees and Virginia M. Sanders. Mol. Cell. Biol., Feb 2009; 29: 675 – 686.

Verification of a topology model of PorT as an integral outer-membrane protein in Porphyromonas gingivalis. Ky-Anh Nguyen, Jasiek Zylicz, Pawel Szczesny, Aneta Sroka, Neil Hunter, and Jan Potempa. Microbiology, Feb 2009; 155: 328 - 337.

*Calcium/calmodulin-dependent phosphorylation of tumor protein D52 on serine residue 136 may be mediated by CAMK2δ6. Catherine S. Chew, Xunsheng Chen, Hanfang Zhang, Eric A. Berg, and Han Zhang. Am J Physiol Gastrointest Liver Physiol, Dec 2008; 295: G1159 - G1172.

*Hematopoietic protein tyrosine phosphatase mediates β₂-adrenergic receptor-induced regulation of p38 MAPK in a B lymphocyte. Jaclyn W. McAlees and Virginia M. Sanders. Mol. Cell. Biol., Dec 2008; 10.1128/MCB.01466-08

Expression of CYP4F2 in human liver and kidney: assessment using targeted peptide antibodies. Hirani V, Yarovoy A, Kozeska A, Magnusson RP, Lasker JM. Arch Biochem Biophys. Oct. 2008 478(1):59-68.

*Phosphorylation of GRK7 by PKA in cone photoreceptor cells is regulated by light. Shoji Osawa, Rebecca Jo and Ellen R. Weiss. J. of Neurochem. Sept. 2008; 107(5): 1314 – 1324.

*Quantitative Comparison of Sarcomeric Phospho-Proteomes of Neonatal and Adult Rat Hearts. Chao Yuan, Quanhu Sheng, Haixu Tang, Yixue Li, Rong Zeng, and R. John Solaro. Am J Physiol Heart Circ Physiol, August 2008; 295: H647-H656.

Mouse maelstrom, a component of nuage, is essential for spermatogenesis and transposon repression in meiosis. Soper SF, van der Heijden GW, Hardiman TC, Goodheart M, Martin SL, de Boer P, Bortvin A. Dev Cell. August 2008 15(2):285-97.

*Phosphorylation of AFAP-110 affects podosome lifespan in A7r5 cells. Andrea Dorfleutner, YoungJin Cho, Deanne Vincent, Jess Cunnick, Hong Lin, Scott A. Weed, Christian Stehlik, and Daniel C. Flynn. J. Cell Sci., Jul 2008; 121: 2394 - 2405.

*Site-specific Androgen Receptor Serine Phosphorylation Linked to Epidermal Growth Factor-dependent Growth of Castration-recurrent Prostate Cancer. Liliana A. Ponguta, Christopher W. Gregory, Frank S. French, and Elizabeth M. Wilson. J. Biol. Chem., Jul 2008; 283: 20989 - 21001.

*Phosphorylation of UT-A1 urea transporter at serines 486 and 499 is important for vasopressin-regulated activity and membrane accumulation. Mitsi A. Blount, Abinash C. Mistry, Otto Fröhlich, S. Russ Price, Guangping Chen, Jeff M. Sands, and Janet D. Klein. Am J Physiol Renal Physiol, Jul 2008; 295: F295 - F299.

Atrial Natriuretic Peptide Frameshift Mutation in Familial Atrial Fibrillation. Denice M. Hodgson-Zingman, Margaret L. Karst, Leonid V. Zingman, Denise M. Heublein, Dawood Darbar, Kathleen J. Herron, Jeffrey D. Ballew, Mariza de Andrade, John C. Burnett, Jr., and Timothy M. Olson. N. Engl. J. Med., July 2008; 359: 158 - 165.

*Inhibition of GLUT4 Translocation by Tbc1d1, a Rab GTPase-activating Protein Abundant in Skeletal Muscle, Is Partially Relieved by AMP-activated Protein Kinase Activation. Jose A. Chavez, William G. Roach, Susanna R. Keller, William S. Lane, and Gustav E. Lienhard. J. Biol. Chem., April 2008; 283: 9187 - 9195.

A Critical Role of Cavin (Polymerase I and Transcript Release Factor) in Caveolae Formation and Organization. Libin Liu and Paul F. Pilch. J. Biol. Chem., February 2008; 283: 4314 - 4322.

*Use of 2-D DIGE analysis reveals altered phosphorylation in a tropomyosin mutant (Glu54Lys) linked to dilated cardiomyopathy. Chad M. Warren, Grace M. Arteaga, Sudarsan Rajan, Rafeeq P. H. Ahmed, David F. Wieczorek and R. John Solaro. Proteomics 2008; 8: 100–105.

Crustacean dopamine receptors: localization and G protein coupling in the stomatogastric ganglion. Merry C. Clark, Reesha Khan and Deborah J. Baro. Journal of Neurochemistry (2008) 104:1006–1019

*I-kappa-B Kinase Phosphorylates the K63 Deubiquitinase A20 To Cause Feedback Inhibition of the NF-kappa-B Pathway. Jessica E. Hutti, Benjamin E. Turk, John M. Asara, Averil Ma, Lewis C. Cantley, and Derek W. Abbott. Molecular and Cellular Biol. (2007) 27(21): 7451-61

*Transducin Translocation in Rods Is Triggered by Saturation of the GTPase-Activating Complex. Ekaterina S. Lobanova, Stella Finkelstein, Hongman Song, Stephen H. Tsang, Ching-Kang Chen, Maxim Sokolov, Nikolai P. Skiba, and Vadim Y. Arshavsky. The Journal of Neuroscience (2007) 27(5):1151–1160

*Phosphorylation of Sp1 in Response to DNA Damage by Ataxia Telangiectasia-Mutated Kinase. Beatrix A. Olofsson, Crystal M. Kelly, Jiyoon Kim, Stephen M. Hornsby, and Jane Azizkhan-Clifford. Mol. Cancer Res., Dec 2007; 5: 1319 - 1330.

*Phosphorylation at S365 is a gatekeeper event that changes the structure of Cx43 and prevents down-regulation by PKC. Joell L. Solan, Lucrecia Marquez-Rosado, Paul L. Sorgen, Perry J. Thornton, Philip R. Gafken, and Paul D. Lampe. J. Cell Biol., Dec 2007; 179: 1301 - 1309.

*A Novel Tyrosine Phosphorylation Site in Protein Kinase D Contributes to Oxidative Stress-mediated Activation. Heike Döppler and Peter Storz. J. Biol. Chem., Nov 2007; 282: 31873 - 31881.

Molecular cloning, phylogeny and localization of AgNHA1: the first Na+/H+ antiporter (NHA) from a metazoan, Anopheles gambiae. Mark R. Rheault, Bernard A. Okech, Stephen B. W. Keen, Melissa M. Miller, Ella A. Meleshkevitch, Paul J. Linser, Dmitri Y. Boudko, and William R. Harvey. J. Exp. Biol., Nov 2007; 210: 3848 - 3861.

*Compartment-specific Phosphorylation of Phosducin in Rods Underlies Adaptation to Various Levels of Illumination. Hongman Song, Marycharmain Belcastro, E. J. Young, and Maxim Sokolov. J. Biol. Chem., Aug 2007; 282: 23613 - 23621.

*Src-dependent Phosphorylation of Membrane Type I Matrix Metalloproteinase on Cytoplasmic Tyrosine 573: ROLE IN ENDOTHELIAL AND TUMOR CELL MIGRATION. Carine Nyalendo, Marisol Michaud, Edith Beaulieu, Christian Roghi, Gilian Murphy, Denis Gingras, and Richard Béliveau. J. Biol. Chem., May 2007; 282: 15690 - 15699.

*Cyclin-dependent kinase 5 modulates nociceptive signaling through direct phosphorylation of transient receptor potential vanilloid 1. Tej K. Pareek, Jason Keller, Sashi Kesavapany, Nitin Agarwal, Rohini Kuner, Harish C. Pant§, Michael J. Iadarola, Roscoe O. Brady, and Ashok B. Kulkarni. Proc. Natl. Acad. Sci. U.S.A. (2007) Jan 9;104(2):660-5

Related to Testes-Specific, Vespid, and Pathogenesis Protein-1 (RTVP-1) Is Overexpressed in Gliomas and Regulates the Growth, Survival, and Invasion of Glioma Cells. Tovit Rosenzweig, Amotz Ziv-Av, Cunli Xiang, Wei Lu, Simona Cazacu, Dvir Taler, Cathie G. Miller, Reuven Reich, Yigal Shoshan, Yair Anikster, Gila Kazimirsky, Ronit Sarid, and Chaya Brodie. Cancer Res. (2006) 66:(8)

Phosphorylation and cleavage of presenilin-associated rhomboid-like protein (PARL) promotes changes in mitochondrial morphology. Danny V. Jeyaraju, Liqun Xu, Marie-Claude Letellier, Sirisha Bandaru, Rodolfo Zunino, Eric A. Berg, Heidi M. McBride, and Luca Pellegrini. Proc. Natl. Acad. Sci., Dec 2006; 103: 18562 - 18567.

*New Role for hPar-1 Kinases EMK and C-TAK1 in Regulating Localization and Activity of Class IIa Histone Deacetylases. Franck Dequiedt, Maud Martin, Julia Von Blume, Didier Vertommen, Emily Lecomte, Nathalie Mari, Marie-France Heinen, Malte Bachmann, Jean-Claude Twizere, Mei Chris Huang, Mark H. Rider, Helen Piwnica-Worms, Thomas Seufferlein, and Richard Kettmann. Mol. Cell. Biol., Oct 2006; 26: 7086 - 7102.

*Integrin-induced Tyrosine Phosphorylation of Protein-tyrosine Phosphatase-α Is Required for Cytoskeletal Reorganization and Cell Migration. Min Chen, Shirley C. Chen, and Catherine J. Pallen. J. Biol. Chem., Apr 2006; 281: 11972 - 11980.

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Selected Scientific Publications From 21^st Century Biochemicals' Customers
Custom Peptides

Antitumor Activity of GSK1904529A, a Small-molecule Inhibitor of the Insulin-like Growth Factor-I Receptor Tyrosine Kinase. Peter Sabbatini, Jason L. Rowand, Arthur Groy, Susan Korenchuk, Qi Liu, Charity Atkins, Melissa Dumble, Jingsong Yang, Kelly Anderson, Brian J. Wilson, Kyle A. Emmitte, Sridhar K. Rabindran, and Rakesh Kumar Clin. Cancer Res., Apr 2009; 10.1158/1078-0432.CCR-08-2530

Auger Radiation–Induced, Antisense-Mediated Cytotoxicity of Tumor Cells Using a 3-Component Streptavidin-Delivery Nanoparticle with ¹¹¹In. Xinrong Liu, Yi Wang, Kayoko Nakamura, Sumi Kawauchi, Ali Akalin, Dengfeng Cheng, Ling Chen, Mary Rusckowski, and Donald J. Hnatowich. J. Nucl. Med., Apr 2009; 50: 582 - 590.

Effects of acyl chain length, unsaturation and pH on thermal stability of model discoidal high-density lipoproteins. Madhumita Guha, Donald L. Gantz, and Olga Gursky. J. Lipid Res., May 2008; 10.1194.

COPI coatomer complex proteins facilitate the translocation of anthrax lethal factor across vesicular membranes in vitro. Alfred G. Tamayo, Ajit Bharti, Carolina Trujillo, Robert Harrison, and John R. Murphy. Proc. Natl. Acad. Sci., Apr 2008; 105: 5254 - 5259.

Human Macrophage Inflammatory Protein 3α: Protein and Peptide Nuclear Magnetic Resonance Solution Structures, Dimerization, Dynamics, and Anti-Infective Properties. David I. Chan, Howard N. Hunter, Brian F. Tack, and Hans J. Vogel. Antimicrob. Agents Chemother., Mar 2008; 52: 883 - 894.

Characterization of an exosite binding inhibitor of matrix metalloproteinase 13. Lata T. Gooljarsingh, Ami Lakdawala, Frank Coppo, Lusong Luo, Gregg B. Fields, Peter J. Tummino, and Richard R. Gontarek. Protein Sci., Jan 2008; 17: 66 - 71.

Sphingosine-1-phosphate induces the association of membrane-type 1 matrix metalloproteinase with p130Cas in endothelial cells. Denis Gingras*, Marisol Michaud, Genevie've Di Tomasso, Eric Be'liveau, Carine Nyalendo, Richard Be'liveau. FEBS Letters (2008) 582:399–404

GTP-independent rapid and slow endocytosis at a central synapse. Jianhua Xu, Benjamin McNeil, Wei Wu, David Nees, Li Bai and Ling-Gang Wu. Nature Neuroscience (2008) 11:1

Cell Culture and Xenograft-Bearing Animal Studies of Radiolabeled Antisense DNA–Carrier. Nanoparticles with Streptavidin as a Linker. Kayoko Nakamura, Yi Wang, Xinrong Liu, Atsushi Kubo, and Donald J. Hnatowich. J. Nucl. Med., Nov 2007; 48: 1845 - 1852.

Novel C5a Agonist-Based Dendritic Cell Vaccine in a Murine Model of Melanoma. Anthony A. Floreani, Sandra J. Gunselman, Arthur J. Heires, Ralph J. Hauke, Stefano Tarantolo, John D. Jackson. Cell Cycle (2007) 6:22, 2835-2839

Protective capacity and epitope specificity of CD8+ T cells responding to lethal West Nile virus infection. James D. Brien, Jennifer L. Uhrlaub and Janko Nikolich-Zˇugich. Eur J Immunol. 2007 Jul;37(7):1855-63

Narrowed TCR repertoire and viral escape as a consequence of heterologous immunity. Markus Cornberg, Alex T. Chen, Lee A. Wilkinson, Michael A. Brehm, Sung-Kwon Kim, Claudia Calcagno, Dario Ghersi, Roberto Puzone, Franco Celada, Raymond M. Welsh, and Liisa K. Selin. J Clin Invest. (2006) 116(5):1443-56

Steady-state Kinetic Mechanism of PDK1. Xinxin Gao and Thomas K. Harris J. Biol. Chem., Aug 2006; 281: 21670 - 21681.

Intracellular Trafficking of KA2 Kainate Receptors Mediated by Interactions with Coatomer Protein Complex I (COPI) and 14-3-3 Chaperone Systems. Pornpun Vivithanaporn, Sheng Yan, and Geoffrey T. Swanson. J. Biol. Chem., Jun 2006; 281: 15475 - 15484.

Megalin binds and internalizes angiotensin-(1-7). Romer Gonzalez-Villalobos, R. Bryan Klassen, Patricia L. Allen, Kelly Johanson, Chasity B. Baker, Hiroyuki Kobori, L. G. Navar, and Timothy G. Hammond. Am J Physiol Renal Physiol. May 2006; 290(5): 1270–1275.

Mechanism of Substrate Recognition by Drug-Resistant Human Immunodeficiency Virus Type 1 Protease Variants Revealed by a Novel Structural Intermediate. Moses Prabu-Jeyabalan, Ellen A. Nalivaika, Keith Romano, and Celia A. Schiffer. J. Virol., Apr 2006; 80: 3607 - 3616.

Th-Cytotoxic T-Lymphocyte Chimeric Epitopes Extended by Ν-ε-Palmitoyl Lysines Induce Herpes Simplex Virus Type 1-Specific Effector CD8 Tc1 Responses and Protect against Ocular Infection. Xiuli Zhang, Annie Issagholian, Eric A. Berg, Jordan B. Fishman, Anthony B. Nesburn, and Lbachir BenMohamed. Journal of Virology (2005) 79(24):15289–15301

Inhibition of colony-stimulating-factor-1 signaling in vivo with the orally bioavailable cFMS kinase inhibitor GW2580. James G. Conway, et al. Proc. Natl. Acad. Sci., Nov 2005; 102: 16078 - 16083.

A conserved motif in transmembrane helix 1 of diphtheria toxin mediates catalytic domain delivery to the cytosol. Ryan Ratts, Carolina Trujillo, Ajit Bharti, Johanna vanderSpek, Robert Harrison, and John R. Murphy. Proc. Natl. Acad. Sci., Oct 2005; 102: 15635 - 15640.