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How Do I Choose a Peptide Sequence?
Peptides can be manufactured for a variety of reasons and applications. Many peptides are used as antigens to produce antibodies to larger proteins, some are used as ligands in receptor binding or competition experiments, and still others are made to search for biological activity or inhibitory ability. No matter what the application, most peptides of biological interest are derived from the N-terminal, C-terminal, or internal sequences of native proteins. In some cases novel sequences are made, but in either case, certain issues arise during peptide synthesis and purification that must be addressed before synthesis. These include having to deal with long stretches of hydrophobic amino acids, cyclizing the peptide to mimic a disulfide bond, or the need to introduce chemical moieties, such as dyes or spin-labeled amino acids, modified amino acids, such as phosphotyrosine, phosphoserine or phosphothreonine, as well as a myriad of other modifications such as biotinylation, acylation, or nitronation. In some cases, changes must be made to insure that the peptide can be solubilized without the need for large amounts of organics, typically avoided due to their incompatibility with biological systems. The technical staff can help to determine the best manner in which to manufacture a peptide to suit your specific needs.
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