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How are antibodies affinity purified?
Antibodies that are specific for the target peptide can be isolated through a technique called affinity purification. Peptide identical to that used for immunization is conjugated to agarose (or other inert) beads that are in turn exposed to serum. Antibodies in the serum specific for the target peptide bind to the peptide-beads and nonspecifically bound proteins are washed away. The specific antipeptide antibodies are eluted from the beads and collected and are termed affinity purified antibodies.
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