How do you measure the antibody titer?
ELISA is used to determine the antibody titer. Specifically designed 96-well plates are coated with BSA-peptide and washed extensively to remove BSA-peptide. Antibody is then added to each well at varying dilutions and allowed to bind to the peptide. The wells are then washed to remove unbound antibody. An enzyme-linked secondary antibody is then used to measure the extent of antibody bound to the plate.


 

What Types of Antigens Can Be Used to Manufacture a Polyclonal Antibody?
Is There an Advantage to Using a Synthetic Peptide to Manufacture My Antibody?
Why Does a Peptide Have To Be Crosslinked to Another Protein?
What temperature I should use to store antibody?
How do you measure the antibody titer?
How are antibodies affinity purified?
Should I Order an Affinity Purified Antibody?

 

 

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